主要从事结构生物学研究，结合生物化学、分子生物学以及细胞生物学等研究手段，着重开展与人源DNA损伤修复、致病微生物肽聚糖代谢以及铁代谢过程中关键蛋白的结构和功能机理研究。

主持科研项目包括：

1. 国家自然科学基金：WT1在DNA损伤应答中的功能及诱导Wilms肿瘤的分子机制（No.81874160）

2. 河北省百人计划 ( No. E2016100014)

3. 国家自然科学基金青年项目《大肠杆菌肽聚糖水解酶LdcA 的结构与功能研究》（No.31300056）

4. 河北省自然科学基金青年基金项目《大肠杆菌肽聚糖水解酶的结构与功能研究》（No.C2014201039）

5. 河北省教育厅优秀青年基金《大肠杆菌O157中肽聚糖水解酶MpaA的结构测定与功能研究》（No.YQ2014007）

1.Xiuhua Liu, Qian Du, ZhiWang, Deyu Zhu, Yan Huang, Ning Li, TiandiWei, Sujuan Xu, Lichuan Gu*. Crystal structure and biochemical features of EfeB/YcdB from Escherichia coli O157: Asp235 plays divergent roles in different enzyme-catalyzed processes. J. Biol. Chem, 2011, 286: 14922-14931. (SCI, IF: 5.38)
2. Guijun Shang, Xiuhua Liu, Defen Lu, Junbing Zhang, Ning Li, Chunyuan Zhu, Shiheng Liu, Qian Yu, Yanyu Zhao, Heqiao Zhang, Junqiang Hu, Huaixing Cang, Sujuan Xu, Lichuan Gu*. Structural insight into how Pseudomonas aeruginosa peptidoglycanhydrolase Tse1 and its immunity protein Tsi1 function. Biochem J, 2012, 448(2): 201-11. (并列第一作者) (SCI, IF: 5.016)
3. Xiuhua Liu, Qian Du, Zhi Wang, Shiheng Liu, Ning Li, Ying Chen, Chunyuan Zhu, Deyu Zhu, Tiandi Wei, Yan Huang, Sujuan Xu, Lichuan Gu*. Crystal Structure of Periplasmic Catecholate-Siderophore Binding Protein VctP from Vibrio cholerae at 1.7 ? Resolution. FEBS Letters, 2012, 586 (8): 1240-1244. (SCI, IF: 3.54)
4. Xiuhua Liu, Jing Gong, Tiandi Wei, Zhi Wang, Qian Du, Deyu Zhu, Yan Huang, Sujuan Xu and Lichuan Gu*. Crystal structure of HutZ, a heme storage protein from Vibrio cholerae: A structural mismatch observed in the region of high sequence conservation. BMC Structural Biology, 2012, 12:23. (SCI, IF: 2.86)
5. Xiuhua Liu, Zhi Wang, Deyu Zhu, Tiandi Wei, Lichuan Gu, Sujuan Xu. Crystallization and preliminary X-ray crystallographic studies of VibE, a vibriobactin-specific 2, 3-dihydroxybenzoate-AMP ligase from Vibrio cholerae. Acta Cryst. F. 2011, 67: 1563-1565 (SCI, IF: 0.56).
6.Shiheng Liu, Conggang Zhang, Ning Li, Bei Niu, Mengyuan Liu, Xiuhua Liu, Tiandi Wei, Deyu Zhu, Yan Huang, Sujuan Xu and Lichuan Gu*. Structural insight into the ISC domain of VibB from Vibrio cholerae at atomic resolution: a snapshot just before the enzymatic reaction. Acta Cryst. D, 2012, 68, 1329-1338. (SCI, IF: 12.619)
7. Bingqing Li, Ning Li, Feng Wang, Liming Guo, Yan Huang, Xiuhua Liu, Tiandi Wei, Deyu Zhu, Cuilan Liu, Hongfang Pan, Sujuan Xu, Hong-Wei Wang and Lichuan Gu*. Structural insight of a concentration-dependent mechanism by which YdiV inhibits Escherichia coli flagellum biogenesis and motility. Nucleic Acids Res, 2012, 40(21): 11073-85. (SCI, IF: 8.02)
8. Ning Li, Conggang Zhang, Bingqing Li, Xiuhua Liu, Yan Huang, Sujuan Xu, and Lichuan Gu. An unique iron coordination in the iron-chelating molecule vibriobactin helps Vibrio cholera evade the mammalian siderocalin-mediated immune response. J. Biol. Chem. 2012, 287(12): 8912-8919 (SCI, IF: 5.38).
9.刘秀华, 刘建凤, 卢雪梅. 纤维弧菌的低温纤维素酶研究, 安徽农业科学, 2012, 40(3): 1281-1282.
10.刘秀华, 王执, 徐素娟. 霍乱弧菌中弧菌素合成酶VibF的A结构域基因的克隆.表达与纯化研究.河南农业科学. 2012, 41 (3): 149-153.
11.卢雪梅.刘秀华《探识生物学》第4卷《微生物》（译著）山东教育出版社，2005年版.

12. Jiaxu Wang, Zenglin Yuan, Yaqi Cui, Rong Xie, Guang Yang, Muzaffer A. Kassab, Mengxi Wang, Yinliang Ma, Chen Wu, Xiaochun Yu* and Xiuhua Liu*. Molecular basis for the inhibition of the methyl-lysine binding function of 53BP1 by TIRR, Nature Communications, 2018, 9: 2689.

13. Mengxi Wang, Zenglin Yuan, Rong Xie, Yinliang Ma, Xiuhua Liu* and Xiaochun Yu*. Structure-function analyses reveal the mechanism of the ARH3-dependent hydrolysis of ADP-ribosylation, Journal of Biological Chemistry, 2018，293(37): 14470–14480.